Neutral a-mannosidase from fungus Aspergillus fischeri (NCIM 508) belongs to Class IIC subfamily

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Year 2012 Vol 1 Issue 4
Document Type : SHORT COMMUNICATION
¹Shashidhara KS1,2* ² Prashanth Kumar HP1 ³ Sameer Srivastava2 ⁴ Gaikwad SM2
¹1Department of Biotechnology, Sapthagiri College of Engineering, Bangalore, Karnataka, 560057 India., 2Biochemical Sciences Division, CSIR-National Chemical Laboraory, Pune, Maharastra, 411008 India
²1Department of Biotechnology, Sapthagiri College of Engineering, Bangalore, Karnataka, 560057 India.,
³2Biochemical Sciences Division, CSIR-National Chemical Laboraory, Pune, Maharastra, 411008 India
⁴2Biochemical Sciences Division, CSIR-National Chemical Laboraory, Pune, Maharastra, 411008 India
Abstract
The a-mannosidase gene from Aspergillus fischeri was isolated by DNA and RNA method

Cloning of gene, isolation of recombinant plasmid and sequencing of gDNA gene was carried out and bioinformatic analysis was done for gene to get intron-exon information and for sequence homology
:The a-mannosidase gene from Aspergillus fischeri is 1990 base pair long and sequence analysis showed presence of three introns and four exons in the gene. BLAST analysis revealed 100% similarity to the Neosartorya fischeri (A. fischeri) and 94% with Aspergillus fumigatus class I a-mannosidase reported in NCBI Gene Data Bank. The neutral a-mannosidase from Aspergillus fischeri could belong to the Class IIC subfamily.
Keywords
: a-mannosidase gene, Aspergillus fischeri, Cloning, Sequencing, gDNA.
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